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vector m o m tm immunodetection kit  (Vector Laboratories)


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    Vector Laboratories vector m o m tm immunodetection kit
    Vector M O M Tm Immunodetection Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+m+o+m+tm+immunodetection+kit/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Basic+Kit/pm41223976-114-18-18
    Average 96 stars, based on 2773 article reviews
    vector m o m tm immunodetection kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: NDRG4, an early detection marker for colorectal cancer, is specifically expressed in enteric neurons.
    Article Snippet: .. In addition, to diminish recognition of endogenous mouse immunoglobins by the mouse primary antibody, slides were subsequently stained with the mouse anti- human NDRG4 antibody, clone 2G3 (1:500; H00065009- M01; Abnova) using the Vector® M.O.M.TM Immunodetection Kit according to manufacturer’s instructions (Vector Labs, Burlingame, CA, USA). ..

    Article Title: Delayed adoptive transfer of bone marrow-derived macrophages modulates post-ischemic inflammation in a model of neonatal hypoxia-ischemia.
    Article Snippet: Samples were fixed with 4 % paraformaldehyde for 30 min. Endogenous peroxidases were blocked with PBS containing 0.3 % hydrogen peroxidase (H2O2, Sigma-Aldrich) and 3 % normal horse serum (Gibco) for 10 min. .. Mouse anti-microtubule-associated protein 2 (MAP-2, 1:1000, Sigma) or myelin basic protein (MBP, 1:1000, Covance) staining was performed using VECTOR® M.O.M.TM Immunodetection Kit according to the manufacturer’s specifications. .. Sections were incubated for 1 h with biotinylated donkey-anti-mouse secondary antibody, and 1 h with VECTASTAIN® Elite ABC-Peroxidase Kit before enzymatic coloration of immunoreactivity was performed by 2-minute immersion in 3,3́-diaminobenzidine (DAB, DAKO).

    Article Title: Leukocyte transmigration in inflamed liver: A role for endothelial cell-selective adhesion molecule.
    Article Snippet: Background/Aims: This study was designed to investigate the role of endothelial cell-selective adhesion molecule (ESAM), a recently discovered receptor expressed in endothelial tight junctions and platelets, for leukocyte migration in inflamed liver.. Methods: The role of ESAM for leukocyte migration in the liver was analyzed using ESAM-deficient mice in a model of warm hepatic ischemia-reperfusion (90 min / 30–360 min).. Results: As shown by immunostaining, ESAM is expressed in sinusoids as well as in venules and is not upregulated upon I / R. Emigrated leukocytes were quantified in tissue sections.

    Immunodetection:

    Article Title: NDRG4, an early detection marker for colorectal cancer, is specifically expressed in enteric neurons.
    Article Snippet: .. In addition, to diminish recognition of endogenous mouse immunoglobins by the mouse primary antibody, slides were subsequently stained with the mouse anti- human NDRG4 antibody, clone 2G3 (1:500; H00065009- M01; Abnova) using the Vector® M.O.M.TM Immunodetection Kit according to manufacturer’s instructions (Vector Labs, Burlingame, CA, USA). ..

    Article Title: Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane.
    Article Snippet: The cells were then incubated with the following antibodies against human antigens rabbit anti-albumin (ALB) at 1:200 (Sigma-Aldrich), rabbit anti-Cytochrome P450 (CYP) 2B6 at 1:50 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-collagen I at 1:100, rabbit anti-fibronectin at 1:200, mouse anti-collagen IV at 1:100, mouse anti-laminin at 1:200 (ZSGB-BIO), mouse anti-multidrug resistancerelated protein 2 (MRP2) at 1:100 (Santa Cruz), mouse anti-hepatocyte nuclear factor (HNF) 4α at 1:50 (Santa Cruz), and mouse anti-human nuclei at 1:1000 (Millipore, Darmstadt, Germany) or rat monoclonal antibody against mouse CD31 at 1:50 (Santa Cruz) at 4 °C overnight. .. A Vector® M.O.M.TM immunodetection kit (Vector Laboratories, Inc., Burlingame, CA, USA) was used according to the manufacturer’s protocol to detect the mouse primary monoclonal antibodies for HNF4α and human nuclei on mouse tissues after hASC-HLC–3D-AHAM transplantation. .. Following three 5-minute washes in PBS with gentle agitation, an Alexa Fluor-conjugated secondary antibody (1:500; Invitrogen) was added, and the samples were incubated for 1 hour at 37 °C.

    Article Title: Pre-Clinical Safety and Efficacy Evaluation of Amytrap, a Novel Therapeutic to Treat Alzheimer’s Disease
    Article Snippet: Subsequently, sections were incubated free-floating in PBS containing Triton X-100 (Sigma, St. Louis, MO), M.O.M.TM protein concentrate (PK-2200, Vector Lab), and a monoclonal mouse anti-6E10 antibody (1 : 5000) for 17 h at 4◦C. .. The immunoreaction product was visualized according to the avidinbiotin complex method with Vector® M.O.M.TM Immunodetection Kit (PK-2200 Kit) as per the manufacturer’s instructions from Vector Lab, Burlington, ON, Canada. .. This was followed by incubation of the sections for 2 min in acetate-imidazole buffer containing nickel sulfate, 3,3′-diaminobenzidine tetrahydrochloride (Sigma), and H2O2.

    Article Title: Delayed adoptive transfer of bone marrow-derived macrophages modulates post-ischemic inflammation in a model of neonatal hypoxia-ischemia.
    Article Snippet: Samples were fixed with 4 % paraformaldehyde for 30 min. Endogenous peroxidases were blocked with PBS containing 0.3 % hydrogen peroxidase (H2O2, Sigma-Aldrich) and 3 % normal horse serum (Gibco) for 10 min. .. Mouse anti-microtubule-associated protein 2 (MAP-2, 1:1000, Sigma) or myelin basic protein (MBP, 1:1000, Covance) staining was performed using VECTOR® M.O.M.TM Immunodetection Kit according to the manufacturer’s specifications. .. Sections were incubated for 1 h with biotinylated donkey-anti-mouse secondary antibody, and 1 h with VECTASTAIN® Elite ABC-Peroxidase Kit before enzymatic coloration of immunoreactivity was performed by 2-minute immersion in 3,3́-diaminobenzidine (DAB, DAKO).

    Article Title: Leukocyte transmigration in inflamed liver: A role for endothelial cell-selective adhesion molecule.
    Article Snippet: Background/Aims: This study was designed to investigate the role of endothelial cell-selective adhesion molecule (ESAM), a recently discovered receptor expressed in endothelial tight junctions and platelets, for leukocyte migration in inflamed liver.. Methods: The role of ESAM for leukocyte migration in the liver was analyzed using ESAM-deficient mice in a model of warm hepatic ischemia-reperfusion (90 min / 30–360 min).. Results: As shown by immunostaining, ESAM is expressed in sinusoids as well as in venules and is not upregulated upon I / R. Emigrated leukocytes were quantified in tissue sections.

    Article Title: Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft.
    Article Snippet: For immunohistochemistry the following antibodies were used; anti-S100A4 (clone 20.1) [18], anti-ALDH1A1, rabbit polyclonal (cat no ab51028), Abcam, Cambridge, UK, anti-CK19 (clone A-53-B/A2), Abcam, anti-CK-14 (clone LL002), Novacostra Labs. Ltd., Newcastle Upon Tyne, UK, anti-CD49f (clone GoH3), BD Biosciences, anti-Ki-67 (clone Ki-67), anti CD44 (clone DF1485) and anti-EpCAM (clone Moc-31), DAKO, Copenhagen, Denmark, .. Immunohistochemistry was performed using the Dako EnVisionTM + System, Peroxidase (DAB) (K4011, Dako, Glostrup, DenmarK) and Dakoautostainer, or VECTOR M.O.M.TM immunodetection Kit (PK-2200, Vector Laboratories). .. Sections were deparaffinized and epitopes unmasked using PT-Link (Dako) and EnVision Flex target retrieval solution, high pH or low pH, and then treated with 0.03% hydrogen peroxide (H2O2) for 5 minutes to block endogenous peroxidase.

    Article Title: Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells.
    Article Snippet: Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies.. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells.. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6.

    Article Title: Sphingosine kinase 1 regulates mucin production via ERK phosphorylation.
    Article Snippet: .. When mouse monoclonal antibody was used, the Vector M.O.M.TM Immunodetection Kit (Vector Laboratories) was used according to the manufacturer’s protocol. ..

    Bioprocessing:

    Article Title: Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane.
    Article Snippet: The cells were then incubated with the following antibodies against human antigens rabbit anti-albumin (ALB) at 1:200 (Sigma-Aldrich), rabbit anti-Cytochrome P450 (CYP) 2B6 at 1:50 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-collagen I at 1:100, rabbit anti-fibronectin at 1:200, mouse anti-collagen IV at 1:100, mouse anti-laminin at 1:200 (ZSGB-BIO), mouse anti-multidrug resistancerelated protein 2 (MRP2) at 1:100 (Santa Cruz), mouse anti-hepatocyte nuclear factor (HNF) 4α at 1:50 (Santa Cruz), and mouse anti-human nuclei at 1:1000 (Millipore, Darmstadt, Germany) or rat monoclonal antibody against mouse CD31 at 1:50 (Santa Cruz) at 4 °C overnight. .. A Vector® M.O.M.TM immunodetection kit (Vector Laboratories, Inc., Burlingame, CA, USA) was used according to the manufacturer’s protocol to detect the mouse primary monoclonal antibodies for HNF4α and human nuclei on mouse tissues after hASC-HLC–3D-AHAM transplantation. .. Following three 5-minute washes in PBS with gentle agitation, an Alexa Fluor-conjugated secondary antibody (1:500; Invitrogen) was added, and the samples were incubated for 1 hour at 37 °C.

    Transplantation Assay:

    Article Title: Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane.
    Article Snippet: The cells were then incubated with the following antibodies against human antigens rabbit anti-albumin (ALB) at 1:200 (Sigma-Aldrich), rabbit anti-Cytochrome P450 (CYP) 2B6 at 1:50 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-collagen I at 1:100, rabbit anti-fibronectin at 1:200, mouse anti-collagen IV at 1:100, mouse anti-laminin at 1:200 (ZSGB-BIO), mouse anti-multidrug resistancerelated protein 2 (MRP2) at 1:100 (Santa Cruz), mouse anti-hepatocyte nuclear factor (HNF) 4α at 1:50 (Santa Cruz), and mouse anti-human nuclei at 1:1000 (Millipore, Darmstadt, Germany) or rat monoclonal antibody against mouse CD31 at 1:50 (Santa Cruz) at 4 °C overnight. .. A Vector® M.O.M.TM immunodetection kit (Vector Laboratories, Inc., Burlingame, CA, USA) was used according to the manufacturer’s protocol to detect the mouse primary monoclonal antibodies for HNF4α and human nuclei on mouse tissues after hASC-HLC–3D-AHAM transplantation. .. Following three 5-minute washes in PBS with gentle agitation, an Alexa Fluor-conjugated secondary antibody (1:500; Invitrogen) was added, and the samples were incubated for 1 hour at 37 °C.

    Blocking Assay:

    Article Title: Leukocyte transmigration in inflamed liver: A role for endothelial cell-selective adhesion molecule.
    Article Snippet: Background/Aims: This study was designed to investigate the role of endothelial cell-selective adhesion molecule (ESAM), a recently discovered receptor expressed in endothelial tight junctions and platelets, for leukocyte migration in inflamed liver.. Methods: The role of ESAM for leukocyte migration in the liver was analyzed using ESAM-deficient mice in a model of warm hepatic ischemia-reperfusion (90 min / 30–360 min).. Results: As shown by immunostaining, ESAM is expressed in sinusoids as well as in venules and is not upregulated upon I / R. Emigrated leukocytes were quantified in tissue sections.

    Incubation:

    Article Title: Leukocyte transmigration in inflamed liver: A role for endothelial cell-selective adhesion molecule.
    Article Snippet: Background/Aims: This study was designed to investigate the role of endothelial cell-selective adhesion molecule (ESAM), a recently discovered receptor expressed in endothelial tight junctions and platelets, for leukocyte migration in inflamed liver.. Methods: The role of ESAM for leukocyte migration in the liver was analyzed using ESAM-deficient mice in a model of warm hepatic ischemia-reperfusion (90 min / 30–360 min).. Results: As shown by immunostaining, ESAM is expressed in sinusoids as well as in venules and is not upregulated upon I / R. Emigrated leukocytes were quantified in tissue sections.

    Immunohistochemistry:

    Article Title: Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft.
    Article Snippet: For immunohistochemistry the following antibodies were used; anti-S100A4 (clone 20.1) [18], anti-ALDH1A1, rabbit polyclonal (cat no ab51028), Abcam, Cambridge, UK, anti-CK19 (clone A-53-B/A2), Abcam, anti-CK-14 (clone LL002), Novacostra Labs. Ltd., Newcastle Upon Tyne, UK, anti-CD49f (clone GoH3), BD Biosciences, anti-Ki-67 (clone Ki-67), anti CD44 (clone DF1485) and anti-EpCAM (clone Moc-31), DAKO, Copenhagen, Denmark, .. Immunohistochemistry was performed using the Dako EnVisionTM + System, Peroxidase (DAB) (K4011, Dako, Glostrup, DenmarK) and Dakoautostainer, or VECTOR M.O.M.TM immunodetection Kit (PK-2200, Vector Laboratories). .. Sections were deparaffinized and epitopes unmasked using PT-Link (Dako) and EnVision Flex target retrieval solution, high pH or low pH, and then treated with 0.03% hydrogen peroxide (H2O2) for 5 minutes to block endogenous peroxidase.

    Binding Assay:

    Article Title: Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells.
    Article Snippet: Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies.. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells.. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6.



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